Tg(Thy1-Brainbow3.1)3Jrs
Transgene Detail

Summary

• Symbol: Tg(Thy1-Brainbow3.1)3Jrs
• Name: transgene insertion 3, Joshua R Sanes
• MGI ID: MGI:5469425
• Synonyms: Brainbow 3.1 line 3, TSOGK3
• Transgene: Tg(Thy1-Brainbow3.1)3Jrs Location: unknown
• Alliance: Tg(Thy1-Brainbow3.1)3Jrs page

Transgene origin

• Strain of Origin: C57BL/6J x CBA

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The Thy1-Brainbow 3.1 transgene was designed by Drs. Dawen Cai and Joshua R.Sanes (Harvard University) with the mouse Thy1 (thymus cell antigen 1, theta) regulatory elements surrounding four fluorescent protein (Yellow fluorescent protein (Phialidium sp); Orange fluorescent protein: Green fluorescent protein; Far-red fluorescent protein) sequences uniquely flanked with pairs of incompatible lox sites. Specifically, this Brainbow 3.1 coding region contained (from 5' to 3') a loxP site, lox2272 site, loxN site, non-fluorescent nuclear localized derivative of phi-YFP (phiNFPnls), polyA sequence, loxP site, farnesylated mOrange2, polyA sequence, lox2272 site, farnesylated EGFP, polyA sequence, loxN site, farnesylated mKate2, polyA sequence. Line 3 was generated. (J:101977, J:197821)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:197821 Cai D, et al., Improved tools for the Brainbow toolbox. Nat Methods. 2013 May 5;10(6):540-7
• All: 3 reference(s)