Pik3ca^tm1Gne
Targeted Allele Detail

Summary

• Symbol: Pik3ca^tm1Gne
• Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; targeted mutation 1, Genentech
• MGI ID: MGI:5469577
• Synonyms: Pik3ca^e20mwt
• Gene: Pik3ca Location: Chr3:32451203-32520256 bp, + strand Genetic Position: Chr3, 15.7 cM, cytoband B
• Alliance: Pik3ca^tm1Gne page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193377
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Single point mutation
• Mutation details: A loxP site was in upstream of the wild-type exon 20, which was followed by a polyadenylation sequence, an FRT-flanked neo cassette, 4x transcriptional STOP, a loxP site and a modified exon 20. The modified exon 20 contained an A to G transition results in the amino acid substitution of arginine for histidine at position 1047 (H1047R). Flp-mediated recombination removed the FRT-flanked neo cassette. Cre-mediated recombination is required to remove the wild-type exon 20 and allow for expression of the modified exon 20. (J:193377)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Pik3ca^tm1Gne

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pik3ca Mutation: 66 strains or lines available

References

• Original: J:193377 Yuan W, et al., Conditional activation of Pik3ca(H1047R) in a knock-in mouse model promotes mammary tumorigenesis and emergence of mutations. Oncogene. 2013 Jan 17;32(3):318-26
• All: 3 reference(s)