Crymtm1b(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Crymtm1b(KOMP)Wtsi |
Name: |
crystallin, mu; targeted mutation 1b, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:5471222 |
Gene: |
Crym Location: Chr7:119785603-119801212 bp, - strand Genetic Position: Chr7, 64.47 cM
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Alliance: |
Crymtm1b(KOMP)Wtsi page
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IMPC: |
Crym gene page |
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Mutant Cell Line: |
EPD0234_3_C05 |
Germline Transmission: |
Earliest citation of germline transmission:
J:188991
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Parent Cell Line: |
JM8.N4 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutations: |
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Insertion, Intragenic deletion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 119800065 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 119800784. The critical exon(s) were thus flanked by loxP sites and subsequent cre expression then excised this critical sequence resulting in a reporter knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.
(J:148605, J:173534, J:188991)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012; |
All: |
7 reference(s) |
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