Cyb5r3^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cyb5r3^{tm1b(KOMP)Wtsi}
• Name: cytochrome b5 reductase 3; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5471295
• Gene: Cyb5r3 Location: Chr15:83037695-83056793 bp, - strand Genetic Position: Chr15, 39.4 cM, cytoband E
• Alliance: Cyb5r3^{tm1b(KOMP)Wtsi} page
• IMPC: Cyb5r3 gene page

Mutation origin

• Mutant Cell Lines: EPD0168_2_A08, EPD0168_2_C07
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 83046309 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 83046870. The critical exon(s) were thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele and subsequent cre expression then excised this critical sequence resulting in a reporter knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:148605, J:173534, J:188991)

Expression

In Mice Carrying this Mutation:	46 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cyb5r3 Mutation: 19 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 5 reference(s)