Mustn1^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mustn1^{tm1a(EUCOMM)Wtsi}
• Name: musculoskeletal, embryonic nuclear protein 1; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:5473008
• Gene: Mustn1 Location: Chr14:30601214-30603567 bp, + strand Genetic Position: Chr14, 19.09 cM
• Alliance: Mustn1^{tm1a(EUCOMM)Wtsi} page
• IMPC: Mustn1 gene page

Mutation origin

• Mutant Cell Lines: EPD0917_3_A03, EPD0917_3_D02, EPD0917_3_F04
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 30602117 of Chromosome 14 upstream of the critical exon 2 and part of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 and part of exon 3 at position 30603225. The critical exon 2 and part of exon 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resuts in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Although homozygous aorta and skeletal muscle show a more than 99.9% reduction in transcript levels, under certain conditions such as proinflammatory stimuli (BaCl2-mediated muscle injurgy-regeneration), an increase in transcript expression is seen indicating that the artificial splice acceptor site is not 100% efficient and allows for gene expression. (J:155845, J:173534, J:346460)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 2 lines available
• Carrying any Mustn1 Mutation: 9 strains or lines available

Notes

Mutant cell line EPD0917_3_F04 was used to create mutant mice which demonstrated germline transmission.

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 5 reference(s)