Dll1^{tm1.1(cre/ERT2)Cle}
Targeted Allele Detail

Summary

• Symbol: Dll1^{tm1.1(cre/ERT2)Cle}
• Name: delta like canonical Notch ligand 1; targeted mutation 1.1, Hans Clevers
• MGI ID: MGI:5473277
• Synonyms: Dll1^GFP-Creert2, Dll1^{GFP-ires-CreERT2}
• Gene: Dll1 Location: Chr17:15587616-15597134 bp, - strand Genetic Position: Chr17, 8.95 cM
• Alliance: Dll1^{tm1.1(cre/ERT2)Cle} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193937
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Dll1^{tm1.1(cre/ERT2)Cle} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Dll1 (MGI:104659)

• Mutation details: Homologous recombination was employed to insert an EGFP-IRES-cre/ERT2 cassette, Frt-flanked neo selection cassette and polyA signal into the ATG codon of exon 1 of Dll1 in ES cells. The neo marker was deleted in vivo by flippase (Flp) expression. GFP expression can be detected by confocal analysis in some mesenchymal cells and blood vessels, and in intestinal epithelial cells at the bottom and higher up in crypts. (J:193937)

Recombinase activity

Activity:	Tissue activity of this recombinase allele MGI has not yet included tissue activity data for this allele in any anatomical systems.
Driver:	Dll1 (mouse) Summary of all recombinase alleles driven by Dll1. Alliance mouse Dll1 gene page

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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ht1	Dll1^tm1.1(cre/ERT2)Cle/Dll1^+	Not Specified

Phenotypes:

Affected Systems	ht1
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normal phenotype	√
no abnormal phenotype detected	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dll1 Mutation: 46 strains or lines available

References

• Original: J:193937 van Es JH, et al., Dll1+ secretory progenitor cells revert to stem cells upon crypt damage. Nat Cell Biol. 2012 Oct;14(10):1099-104
• All: 4 reference(s)