Col1a1^{tm2(tetO-CTNNB1*)Hoch}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm2(tetO-CTNNB1*)Hoch}
• Name: collagen, type I, alpha 1; targeted mutation 2, Konrad Hochedlinger
• MGI ID: MGI:5473958
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm2(tetO-CTNNB1*)Hoch} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:191050
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm2(tetO-CTNNB1*)Hoch} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ctnnb1 (MGI:88276)

• Mutation details: RMCE was used to insert a human beta-catenin sequence containing an S33Y codon substitution under the control of a tetO and a minimal promoter. Insertion was in the 3' non coding region. (J:191050)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm2(tetO-Pou5f1)Jae} (J:159351).

References

• Original: J:191050 Hirata A, et al., Dose-dependent roles for canonical Wnt signalling in de novo crypt formation and cell cycle properties of the colonic epithelium. Development. 2013 Jan 1;140(1):66-75
• All: 3 reference(s)