Cd3etm1.1Geno
Targeted Allele Detail
|
Symbol: |
Cd3etm1.1Geno |
Name: |
CD3 antigen, epsilon polypeptide; targeted mutation 1.1, Genoway |
MGI ID: |
MGI:5474199 |
Synonyms: |
KI-PRS |
Gene: |
Cd3e Location: Chr9:44910038-44920925 bp, - strand Genetic Position: Chr9, 24.84 cM
|
Alliance: |
Cd3etm1.1Geno page
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:192604
|
Parent Cell Line: |
Not Specified (ES Cell)
|
Strain of Origin: |
Not Specified
|
|
Allele Type: |
|
Targeted (Not Specified) |
Mutations: |
|
Insertion, Nucleotide substitutions
|
|
|
Mutation details: An FRT flanked neo cassette was inserted into intron 6 (intron 4 in the paper) and two C to G mutations were inserted into exon 7 (coding exon 6; exon 5 in the paper) via homologous recombination. These mutations replace two central prolines in the proline-rich sequence with alanines (P163A, P166A). Interaction between the mutant protein and the SH3.1 domain of Nck1 is not seen in a pull down assay. Flp mediated recombination removed the neo cassette.
(J:192604)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
Carrying any Cd3e Mutation: |
54 strains or lines available
|
|
Original: |
J:192604 Borroto A, et al., Nck Recruitment to the TCR Required for ZAP70 Activation during Thymic Development. J Immunol. 2013 Feb 1;190(3):1103-12 |
All: |
5 reference(s) |
|