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Cd3etm1.1Geno
Targeted Allele Detail
Summary
Symbol: Cd3etm1.1Geno
Name: CD3 antigen, epsilon polypeptide; targeted mutation 1.1, Genoway
MGI ID: MGI:5474199
Synonyms: KI-PRS
Gene: Cd3e  Location: Chr9:44910038-44920925 bp, - strand  Genetic Position: Chr9, 24.84 cM
Alliance: Cd3etm1.1Geno page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:192604
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Not Specified)
Mutations:    Insertion, Nucleotide substitutions
 
Mutation detailsAn FRT flanked neo cassette was inserted into intron 6 (intron 4 in the paper) and two C to G mutations were inserted into exon 7 (coding exon 6; exon 5 in the paper) via homologous recombination. These mutations replace two central prolines in the proline-rich sequence with alanines (P163A, P166A). Interaction between the mutant protein and the SH3.1 domain of Nck1 is not seen in a pull down assay. Flp mediated recombination removed the neo cassette. (J:192604)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cd3e Mutation:  54 strains or lines available
References
Original:  J:192604 Borroto A, et al., Nck Recruitment to the TCR Required for ZAP70 Activation during Thymic Development. J Immunol. 2013 Feb 1;190(3):1103-12
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory