Tg(Mchr1-cre)1Emf
Transgene Detail

Summary

• Symbol: Tg(Mchr1-cre)1Emf
• Name: transgene insertion 1, Eleftheria Maratos-Flier
• MGI ID: MGI:5474277
• Synonyms: Mchr1-cre BAC tg
• Transgene: Tg(Mchr1-cre)1Emf Location: unknown
• Alliance: Tg(Mchr1-cre)1Emf page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Mchr1-cre)1Emf expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mchr1 (MGI:2180756)

• Mutation details: A cre recombinase sequence (1032 bp) was inserted downstream of the Mchr1 promoter on the RP23-202N16 BAC via homologous recombination/BAC recombineering; this replaced nucleotides 4-41 from exon 1 and was designed to disable transcription/translation of the Mchr1 protein from the BAC Mchr1 locus. An frt-flanked kanamycin selection cassette used during homologous recombination/BAC recombineering was removed by transient FLP plasmid injection. The resulting ~198 kbp Mchr1-cre BAC transgene was injected into C57BL/6J embryos to establish transgenic founder animals. Founder animals were bred to C57BL/6J mice to generate independent founder lines. One founder line was chosen. (J:194588)

Schematic of the Tg(Mchr1-cre)1Emf transgene

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mchr1 (mouse) Summary of all recombinase alleles driven by Mchr1. Comparative matrix view of recombinase activities Alliance mouse Mchr1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:194588 Chee MJ, et al., Neurochemical charaterization of neurons expressing melanin-concentrating hormone receptor 1 in the mouse hypothalamus. J Comp Neurol. 2013 Jul;521(10):2208-34
• All: 2 reference(s)