Nhej1^tm1.1Ics
Targeted Allele Detail

Summary

• Symbol: Nhej1^tm1.1Ics
• Name: non-homologous end joining factor 1; targeted mutation 1.1, Mouse Clinical Institute
• MGI ID: MGI:5475156
• Synonyms: Cernu^-
• Gene: Nhej1 Location: Chr1:75006505-75101870 bp, - strand Genetic Position: Chr1, 38.56 cM, cytoband C3
• Alliance: Nhej1^tm1.1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194422
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 4. An FRT- and loxP-flanked neo cassette was inserted downstream of exon 4. Cre-mediated recombination removed exon 4 and the neo cassette. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts. (J:194422)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nhej1 Mutation: 16 strains or lines available

References

• Original: J:194422 Vera G, et al., Cernunnos deficiency reduces thymocyte life span and alters the T cell repertoire in mice and humans. Mol Cell Biol. 2013 Feb;33(4):701-11
• All: 4 reference(s)