Cd70^tm1.1Jda
Targeted Allele Detail

Summary

• Symbol: Cd70^tm1.1Jda
• Name: CD70 antigen; targeted mutation 1.1, Jonathan D Ashwell
• MGI ID: MGI:5475220
• Gene: Cd70 Location: Chr17:57452997-57456777 bp, - strand Genetic Position: Chr17, 29.69 cM, cytoband C-D
• Alliance: Cd70^tm1.1Jda page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193042
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129 x C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo cassette was inserted upstream of exon 1. The neo cassette was then excised in properly targeted cells by cre recombinase. A cassette composed of an FRT flanked neo and another loxP site was then inserted downstream of exon 2. This second neo cassette was then removed by crossing to mice carrying Tg(ACTFLPe)9205 Dym. Subsequently, mice were crossed to Tg(ACTB-cre)2Mrt mice to excise exons 1 and 2. Bone marrow derived dendritic cells stimulated with lipopolysaccharide fail to produce mutant mRNA and no protein is expressed on the surface of stimulated splenocytes. (J:193042)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cd70 Mutation: 12 strains or lines available

References

• Original: J:193042 Munitic I, et al., CD70 deficiency impairs effector CD8 T cell generation and viral clearance but is dispensable for the recall response to lymphocytic choriomeningitis virus. J Immunol. 2013 Feb 1;190(3):1169-79
• All: 6 reference(s)