Gclc^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gclc^{tm1c(EUCOMM)Wtsi}
• Name: glutamate-cysteine ligase, catalytic subunit; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5475270
• Synonyms: Gclc^fl
• Gene: Gclc Location: Chr9:77661817-77701767 bp, + strand Genetic Position: Chr9, 43.36 cM, cytoband D-E
• Alliance: Gclc^{tm1c(EUCOMM)Wtsi} page
• IMPC: Gclc gene page

Cataracts in Gclc^{tm1c(EUCOMM)Wtsi}/Gclc^{tm1c(EUCOMM)Wtsi} Tg(Cryaa-cre)10Mlr/0 mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: EPD0051_3_D07
• Germline Transmission: Earliest citation of germline transmission: J:194778
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 77681930 of Chromosome 9 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 2 at position 77682710. The critical exon 2 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ/neo cassette and left exon 2 floxed. (J:194778)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gclc Mutation: 20 strains or lines available

References

• Original: J:194778 Fan X, et al., The LEGSKO mouse: a mouse model of age-related nuclear cataract based on genetic suppression of lens glutathione synthesis. PLoS One. 2012;7(11):e50832
• All: 10 reference(s)