Tg(tetO-Spp1)5Gad
Transgene Detail

Summary

• Symbol: Tg(tetO-Spp1)5Gad
• Name: transgene insertion 5, Alain Gadeau
• MGI ID: MGI:5475548
• Synonyms: Tet-OPN, Tg(TetO-Spp1)5, TRE-rOPN
• Transgene: Tg(tetO-Spp1)5Gad Location: unknown
• Alliance: Tg(tetO-Spp1)5Gad page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F2

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: This transgene contains a tetracycline response element, comprising seven copies of the 42-bp tet operator sequence, between two minimal cytomegalovirus promoters, one driving expression of the E. coli lacZ (beta-galactosidase) gene and the other of a cDNA encoding mouse secreted phosphoprotein 1 (also called osteopontin) (nt 9-955, GenBank accession BC057858). Both genes are inactive except in the presence of a second transgene expressing the tetracycline transactivator (tTA) or reverse transactivator (rtTA) protein, which permits regulation of their expression by administration/discontinuation of doxycycline. (J:159799)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:159799 Leen LL, et al., Estrogen-stimulated endothelial repair requires osteopontin. Arterioscler Thromb Vasc Biol. 2008 Dec;28(12):2131-6
• All: 3 reference(s)