Tg(Itgax-EGFP,-cre,-HBEGF,-luc)2Gjh
Transgene Detail

Summary

• Symbol: Tg(Itgax-EGFP,-cre,-HBEGF,-luc)2Gjh
• Name: transgene insertion 2, Gunter J Hammerling
• MGI ID: MGI:5476442
• Synonyms: CD11c.LuciDTR
• Transgene: Tg(Itgax-EGFP,-cre,-HBEGF,-luc)2Gjh Location: unknown
• Alliance: Tg(Itgax-EGFP,-cre,-HBEGF,-luc)2Gjh page

Transgene origin

• Strain of Origin: C57BL/6N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Recombinase)
• Mutation: Insertion
• Tg(Itgax-EGFP,-cre,-HBEGF,-luc)2Gjh expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HBEGF (1839)

  Hbegf

  (MGI:96070)

• Tg(Itgax-EGFP,-cre,-HBEGF,-luc)2Gjh expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Itgax (MGI:96609)

• Mutation details: A cDNA construct encoding EGFP, cre recombinase, human diphtheria toxin receptor (DTR; HBEGF) and click beetle green 99 (CBG99) luciferase was generated, with coding sequences separated by self-cleaving 2A viral sequences. The presence of 2A sequences results in stoichiometric translation of the individual proteins. This construct was inserted at the ATG start codon of Itgax in the RPCI-24-7812 bacterial artificial chromosome by recombineering in bacterial cells. The modified BAC was linearized and injected into pronuclei of fertilized C57BL/6N oocytes and line 2 was established. (J:194800)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Itgax (mouse) Summary of all recombinase alleles driven by Itgax. Alliance mouse Itgax gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:194800 Tittel AP, et al., Functionally relevant neutrophilia in CD11c diphtheria toxin receptor transgenic mice. Nat Methods. 2012 Apr;9(4):385-90
• All: 2 reference(s)