Mlana^tm1.2Bee
Targeted Allele Detail

Summary

• Symbol: Mlana^tm1.2Bee
• Name: melan-A; targeted mutation 1.2, Friedrich Beermann
• MGI ID: MGI:5476663
• Synonyms: MART-1^-, Mlana^ko
• Gene: Mlana Location: Chr19:29675224-29686034 bp, + strand Genetic Position: Chr19, 24.28 cM, cytoband C2
• Alliance: Mlana^tm1.2Bee page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194886
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The targeting construct introduced loxP sites flanking the second and third exons and a frt-flanked neomycin resistance cassette (Pgk-neo) immediately upstream of the first loxP site, in the first intron. Mice bearing the resultant Mlana^tm1Bee allele were crossed sequentially to FLP and Cre recombinase-expressing mice to delete the Pgk-neo cassette (producing Mlana^tm1.1Bee) and the loxP-flanked exons, respectively. The deleted exons 2, containing the translation initiating ATG, and 3 together encode the transmembrane domain of the protein. Reverse transcription-polymerase chain reaction (RT-PCR) analysis demonstrated the absence of Mlana transcripts in skin of mice homozygous for the present mutation. No MLANA protein was detected either by western blot analysis of extracts of melanocytes cultured from the mutant mice or by immunohistologic analysis of these melanocytes or of skin sections using antibodies to the protein's C-terminus. (J:194886)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mlana Mutation: 9 strains or lines available

References

• Original: J:194886 Aydin IT, et al., Coat color dilution in mice because of inactivation of the melanoma antigen MART-1. Pigment Cell Melanoma Res. 2012 Jan;25(1):37-46
• All: 1 reference(s)