Sumo1^tm1.1Bros
Targeted Allele Detail

Summary

• Symbol: Sumo1^tm1.1Bros
• Name: small ubiquitin-like modifier 1; targeted mutation 1.1, Nils Brose
• MGI ID: MGI:5476974
• Synonyms: His6-HA-SUMO1
• Gene: Sumo1 Location: Chr1:59678593-59709993 bp, - strand Genetic Position: Chr1, 30.32 cM, cytoband C2-C3
• Alliance: Sumo1^tm1.1Bros page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193101
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutation: Insertion Mutation details: Sequence encoding for 6 histidines, a trombine cleavage site and a hemagglutinin (HA) tag was inserted in exon 1 in frame 3' of the start codon and a floxed neo cassette was inserted in intron 1 via homologous recombination. Cre mediated recombination removed the neo cassette. A fusion protein is detected in heterozygous mice. Presence of the tag does not appear to effect substrate specificity of SUMO1 conjugation. (J:193101)

Generation of the Sumo1^tm1.1Bros allele

Expression

In Mice Carrying this Mutation:

16 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sumo1 Mutation: 66 strains or lines available

References

• Original: J:193101 Tirard M, et al., In vivo localization and identification of SUMOylated proteins in the brain of His6-HA-SUMO1 knock-in mice. Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):21122-7
• All: 3 reference(s)