Tbc1d4^tm1.2Arte
Targeted Allele Detail

Summary

• Symbol: Tbc1d4^tm1.2Arte
• Name: TBC1 domain family, member 4; targeted mutation 1.2, TaconicArtemis
• MGI ID: MGI:5478502
• Gene: Tbc1d4 Location: Chr14:101679796-101846627 bp, - strand Genetic Position: Chr14, 50.9 cM
• Alliance: Tbc1d4^tm1.2Arte page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194026
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 10 was replaced with an FRT-flanked neo cassette with a 5' loxP site and a modified exon 10 in which nucleotide substitutions result in the amino acid substitution of alanine for threonine at position 649 (T649A) followed by a loxP site. Flp-mediate recombination removed the neo cassette. Cre-mediated recombination removed the modified exon 10. Western blot analysis confirmed the absence of protein expression in the soleus, gastrocnemius, tibialis anterior, adipose and heart. (J:194026)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbc1d4 Mutation: 51 strains or lines available

References

• Original: J:194026 Wang HY, et al., AS160 deficiency causes whole-body insulin resistance via composite effects in multiple tissues. Biochem J. 2013 Jan 15;449(2):479-89
• All: 3 reference(s)