Pcna^tm1.1Scwh
Targeted Allele Detail

Summary

• Symbol: Pcna^tm1.1Scwh
• Name: proliferating cell nuclear antigen; targeted mutation 1.1, Shao-Chun Wang
• MGI ID: MGI:5478543
• Synonyms: PCNA^114F
• Gene: Pcna Location: Chr2:132091206-132095100 bp, - strand Genetic Position: Chr2, 64.15 cM
• Alliance: Pcna^tm1.1Scwh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193874
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: FVB

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: A loxP site was inserted upstream of exon 2. Exon 3 was replaced with a modified exon 3 in which nucleotide substitution (A to T) that results in the amino acid substitution of phenylalanine for tyrosine at position 114 (Y114F). Flp-mediated recombination removed the FRT-flanked neo cassette with a 3' loxP site inserted downstream of exon 4. Exons 2 through 4 remain floxed. (J:193874)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcna Mutation: 19 strains or lines available

References

• Original: J:193874 Lo YH, et al., Phosphorylation at tyrosine 114 of Proliferating Cell Nuclear Antigen (PCNA) is required for adipogenesis in response to high fat diet. Biochem Biophys Res Commun. 2013 Jan 4;430(1):43-8
• All: 1 reference(s)