Stk11^tm1.1Gne
Targeted Allele Detail

Summary

• Symbol: Stk11^tm1.1Gne
• Name: serine/threonine kinase 11; targeted mutation 1.1, Genentech
• MGI ID: MGI:5484538
• Synonyms: Lkb1^M129G, Lkb1^MG
• Gene: Stk11 Location: Chr10:79951637-79966516 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Stk11^tm1.1Gne page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:195229
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 3 was replaced with a modified exon 3 in which nucleotide substitutions replaces a methionine with glycine at position 129 (M129G). This mutation is expected to disrupt the gatekeeper residue in the ATP-binding pocket possibly rendering the protein susceptible to small molecular analogues of the general kinase inhibitor PP1. Cre-mediated recombination removed the floxed neo cassette inserted downstream of exon 2. Activity assay confirmed the sensitivity to 1NMPP1 inhibition. (J:195229)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stk11 Mutation: 35 strains or lines available

References

• Original: J:195229 Lo B, et al., Lkb1 regulates organogenesis and early oncogenesis along AMPK-dependent and -independent pathways. J Cell Biol. 2012 Dec 24;199(7):1117-30
• All: 1 reference(s)