Snap25^tm1.1Hze
Targeted Allele Detail

Summary

• Symbol: Snap25^tm1.1Hze
• Name: synaptosomal-associated protein 25; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5484607
• Synonyms: Ai52, Ai52D, Snap25-2A, Snap25-LSL-2A-EGFP, Snap25-LSL-2A-EGFP-Delta, Snap25-LSL-2A-EGFP-DeltaNeo/Hygro, Snap25^{tm1.1(xstpx-EGFP)Hze}
• Gene: Snap25 Location: Chr2:136555373-136624348 bp, + strand Genetic Position: Chr2, 67.56 cM
• Alliance: Snap25^tm1.1Hze page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector contained, from 5' to 3', a partial Snap25 sequence spanning intron 6 through intron 7, an frt3 site within Snap25 intron 7, a partial Snap25 exon 8 sequence up to (but not including) the endogenous stop codon, a loxP-flanked STOP cassette that is in-frame with the upstream Snap25 exon 8 sequence (contains translational stop codons in all three reading frames and the PGK polyA sequence), an in-frame F2A peptide cleavage signal, an in frame EGFP gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA signal, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 late polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequences (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL). (J:101977, J:219930)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Snap25 Mutation: 26 strains or lines available

References

• Original: J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58
• All: 9 reference(s)