Gt(ROSA)26Sor^{tm1.1(CAG-EGFP/Rpl10a,-birA)Wtp}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-EGFP/Rpl10a,-birA)Wtp}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, William T Pu
• MGI ID: MGI:5484612
• Synonyms: Gt(ROSA)26Sor^{tm1.1(CAG-EGFP/Rpl10a,birA)Wtp}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-EGFP/Rpl10a,-birA)Wtp} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The fs-TRAP targeting vector was designed with, from 5' to 3', a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a floxed-neomycin (neo) resistance cassette and polyadenylation (polyA) sequence, a frt-flanked enhanced green fluorescence protein (EGFP)-L10a (ribosomal protein) fusion protein, a 2xHA (hemagglutinin) tagged bacterial birA, bifunctional protein (biotin ligase), gene, and a polyA sequence. This targeting vector was inserted into the Gt(ROSA)26Sor gene. Cre-mediated recombination removed the neo cassette. (J:101977)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:201159 Zhou P, et al., Interrogating translational efficiency and lineage-specific transcriptomes using ribosome affinity purification. Proc Natl Acad Sci U S A. 2013 Sep 17;110(38):15395-400
• All: 8 reference(s)