Tac1^{tm1.1(cre)Hze}
Targeted Allele Detail

Summary

• Symbol: Tac1^{tm1.1(cre)Hze}
• Name: tachykinin 1; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5484636
• Synonyms: Tac1-IRES2-Cre, Tac1-IRES2-Cre-Delta, Tac1-IRES2-Cre-DeltaNeo/Hygro, Tac1-IRES2-Cre-DeltaNeo/HygroTac1-IRES2-Cre-D
• Gene: Tac1 Location: Chr6:7555061-7562978 bp, + strand Genetic Position: Chr6, 3.31 cM
• Alliance: Tac1^{tm1.1(cre)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:195475
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Tac1^{tm1.1(cre)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tac1 (MGI:98474)

• Mutation details: The targeting vector contained, from 5' to 3', a partial Tac1 sequence spanning intron 4 through intron 6, an frt3 site within Tac1 intron 6, a partial Tac1 exon 7 sequence including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of a mRNA sequence), a cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 late polyA signal, and an AttP site. This construct was electroporated into (129S6/SvEvTac x C57BL/6)F1-derived G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice (JAX Stock No. 007743) to remove the AttB/AttP-flanked sequences. (J:195475, J:220523)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tac1 (mouse) Summary of all recombinase alleles driven by Tac1. Comparative matrix view of recombinase activities Alliance mouse Tac1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tac1 Mutation: 41 strains or lines available

References

• Original: J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
• All: 62 reference(s)