Tg(Spo11-cre)1Rsw
Transgene Detail

Summary

• Symbol: Tg(Spo11-cre)1Rsw
• Name: transgene insertion 1, Robert S Weiss
• MGI ID: MGI:5484745
• Synonyms: Spo11-cre, Spo11-eGFP-Cre
• Transgene: Tg(Spo11-cre)1Rsw Location: unknown
• Alliance: Tg(Spo11-cre)1Rsw page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Spo11-cre)1Rsw expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Spo11

• Mutation details: An IRES-EGFP-cre cassette and FRT-flanked neo marker were inserted into a BAC containing the Spo11 gene. A fragment containing the Spo11 sequence with 5 kb of upstream sequence, the IRES-EGFP-cre cassette and neo marker was isolated from the modified BAC and transfected into ES cells. Targeted ES cells were injected into blastocysts to generate transgenic animals. Founder information and line numbers are not available. (J:195191)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Spo11 (species not specified) Summary of all recombinase alleles driven by Spo11.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

The initial report indicated that the FRT-flanked neomycin cassette had been removed, however, further sequencing confirmed the transgene had retained the FRT-flanked neo and that it was a 34 bp minimal frt site at the 5' end and a 48 bp frt site at the 3' end.

References

• Original: J:195191 Lyndaker AM, et al., Conditional inactivation of the DNA damage response gene Hus1 in mouse testis reveals separable roles for components of the RAD9-RAD1-HUS1 complex in meiotic chromosome maintenance. PLoS Genet. 2013 Feb;9(2):e1003320
• All: 13 reference(s)