E2f1^{Tg(Wnt1-cre)2Sor}
Transgenic Allele Detail

Summary

• Symbol: E2f1^{Tg(Wnt1-cre)2Sor}
• Name: E2F transcription factor 1; transgene insertion 2, Philippe Soriano
• MGI ID: MGI:5485027
• Synonyms: Tg(Wnt1-cre)2Sor, Wnt1-Cre, Wnt1-Cre2
• Gene: E2f1 Location: Chr2:154401327-154411812 bp, - strand Genetic Position: Chr2, 76.79 cM
• Alliance: E2f1^{Tg(Wnt1-cre)2Sor} page

Transgene origin

• Strain of Origin: C57BL/6 x C3H

Transgene description

• Transgene Type: Transgenic (Null/knockout, Recombinase)
• Mutations: Insertion, Intragenic deletion, Inversion
• E2f1^{Tg(Wnt1-cre)2Sor} expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Wnt1 (MGI:98953)

• Mutation details: A transgenic insert containing cre recombinase, under the control of the 1.3 kb 5' promoter and 5.5 kb 3' enhancer of the mouse Wnt1, wingless-related MMTV integration site 1 was injected into fertilized B6C3 hybrid mouse eggs. Founder line 2 inserted into the gene at 154561346-154561603 (Build GRCm38/mm10) resulting in a 257 bp deletion and a 45 Kb inverted segment in exon 5 of the gene. The inversion contains all of exon 5, but deletes 23 Kb including exons 6 and 7. Founder line 2 has a copy number of 1-3. (J:195690)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Wnt1 (mouse) Summary of all recombinase alleles driven by Wnt1. Comparative matrix view of recombinase activities Alliance mouse Wnt1 gene page

Disease models

Expression

In Mice Carrying this Mutation:

170 assay results 10 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any E2f1 Mutation: 28 strains or lines available

References

• Original: J:199477 Lewis AE, et al., The widely used Wnt1-Cre transgene causes developmental phenotypes by ectopic activation of Wnt signaling. Dev Biol. 2013 Jul 15;379(2):229-34
• All: 137 reference(s)