Col1a1^{tm1(tetO-EWSR1/ATF1)Yasu}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-EWSR1/ATF1)Yasu}
• Name: collagen, type I, alpha 1; targeted mutation 1, Yasuhiro Yamada
• MGI ID: MGI:5485192
• Synonyms: EWS/ATF1
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-EWSR1/ATF1)Yasu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194505
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Humanized sequence, Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-EWSR1/ATF1)Yasu} expresses 2 genes
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ATF1 (466)

  Atf1	(MGI:1298366)
  Atf1-ps	(MGI:3037720)

  human

  EWSR1 (2130)

  Ewsr1

  (MGI:99960)

• Col1a1^{tm1(tetO-EWSR1/ATF1)Yasu} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: RMCE knocked in the construct containing a tetracycline responsive element driving inducible expression of the human fusion sequence from CCS cell line KAS with a FLAG and HA tag into the 3' UTR. (J:194505)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm2(tetO-Pou5f1)Jae}.

References

• Original: J:194505 Yamada K, et al., EWS/ATF1 expression induces sarcomas from neural crest-derived cells in mice. J Clin Invest. 2013 Feb;123(2):600-10
• All: 1 reference(s)