Ghrl^{tm2.1(cre/EGFP)Suss}
Targeted Allele Detail

Summary

• Symbol: Ghrl^{tm2.1(cre/EGFP)Suss}
• Name: ghrelin; targeted mutation 2.1, Lori Sussel
• MGI ID: MGI:5485215
• Synonyms: Ghrl-Cre, grehlin^Cre-EGFP
• Gene: Ghrl Location: Chr6:113693080-113696841 bp, - strand Genetic Position: Chr6, 52.84 cM, cytoband E3
• Alliance: Ghrl^{tm2.1(cre/EGFP)Suss} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:195663
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (RMCE-ready, Recombinase, Reporter)
• Mutations: Insertion, Intragenic deletion
• Ghrl^{tm2.1(cre/EGFP)Suss} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ghrl (MGI:1930008)

• Mutation details: A vector for recombinase-mediated cassette exchange contained the with the sequence encoding a cre/EGFP fusion protein was inserted into a vector containing the region of Ghrl (from upstream of exon 1 to downstream of exon 5) that had been replaced to make the Ghrl^tm1Suss LCA allele. The cre/EGFP open reading frame was inserted at the endogenous start codon. The vector was coinjected with a cre expressing plasmid into ES cells carrying the Ghrl^tm1Suss allele, and the selection cassettes in the LCA allele were replaced with the modified Ghrl sequence. ES cells were injected into blastocysts to generate chimeras that were bred to wild-type Black Swiss mice to obtain germline transmission. These mice were crossed to Tg(ACTBFLPe)9205Dym mice to remove the hygro marker included with the cre/EGFP sequence. (J:195663)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ghrl (mouse) Summary of all recombinase alleles driven by Ghrl. Alliance mouse Ghrl gene page

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ghrl Mutation: 12 strains or lines available

References

• Original: J:195663 Arnes L, et al., Ghrelin expression in the mouse pancreas defines a unique multipotent progenitor population. PLoS One. 2012;7(12):e52026
• All: 3 reference(s)