Gt(ROSA)26Sor^tm1(creB)Dwi
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm1(creB)Dwi
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Douglas J Winton
• MGI ID: MGI:5486456
• Synonyms: Rosa26-creB
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm1(creB)Dwi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194841
• Parent Cell Line: S6B6 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Gt(ROSA)26Sor^tm1(creB)Dwi expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gt(ROSA)26Sor (MGI:104735)

• Mutation details: A cassette was constructed with the creB fragment of cre recombinase (codons 60-343) fused to an FKBP dimerization domain by an F2 linker and included a polyadenylation sequence. This cassette was cloned into a plasmid containing Gt(ROSA)26Sor homology sequences. The linearized plasmid was introduced into ES cells and by homologous recombination the creB sequence was inserted into the Gt(ROSA)26Sor locus. When crossed to mice expressing the creA sequence, the two fragments will dimerize in presence of AP20187 and a functional cre protein will be produced. (J:194841)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gt(ROSA)26Sor (mouse) Summary of all recombinase alleles driven by Gt(ROSA)26Sor. Alliance mouse Gt(ROSA)26Sor gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:194841 Buczacki SJ, et al., Intestinal label-retaining cells are secretory precursors expressing Lgr5. Nature. 2013 Mar 7;495(7439):65-9
• All: 1 reference(s)