Gjb6^tm1.1Mcsa
Targeted Allele Detail

Summary

• Symbol: Gjb6^tm1.1Mcsa
• Name: gap junction protein, beta 6; targeted mutation 1.1, Martine Cohen-Salmon
• MGI ID: MGI:5486677
• Synonyms: Cx30^delta
• Gene: Gjb6 Location: Chr14:57360760-57370764 bp, - strand Genetic Position: Chr14, 30.1 cM
• Alliance: Gjb6^tm1.1Mcsa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193913
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 3 and a floxed pgk-hygromycin cassette was inserted downstream of exon 3 via homologous recombination. Cre mediated recombination removed exon 3 and the pgk-hygromycin cassette. Quantitative PCR and western blot analysis indicate the absence of mRNA and protein expression in the inner ear of homozygous mice. Expression of Gjb2 mRNA and protein are reduced in the inner ear of homozygous mice but to a lesser extent than in mice homozygous for Gjb6^tm1Kwi. (J:193913)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gjb6 Mutation: 20 strains or lines available

References

• Original: J:193913 Boulay AC, et al., Hearing is normal without connexin30. J Neurosci. 2013 Jan 9;33(2):430-4
• All: 5 reference(s)