Nrcam^{tm2e.1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nrcam^{tm2e.1(KOMP)Wtsi}
• Name: neuronal cell adhesion molecule; targeted mutation 2e.1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5488665
• Gene: Nrcam Location: Chr12:44375668-44648747 bp, + strand Genetic Position: Chr12, 20.71 cM
• Alliance: Nrcam^{tm2e.1(KOMP)Wtsi} page
• IMPC: Nrcam gene page

Mutation origin

• Mutant Cell Line: EPD0479_4_E10
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 44596221 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette created a reporter knockout mouse, and subsequent cre expression excised the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:148605, J:173534, J:188991)

Expression

In Mice Carrying this Mutation:

11 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nrcam Mutation: 87 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 7 reference(s)