Arc^{tm1.1(cre/ERT2)Luo}
Targeted Allele Detail

Summary

• Symbol: Arc^{tm1.1(cre/ERT2)Luo}
• Name: activity regulated cytoskeletal-associated protein; targeted mutation 1.1, Liqun Luo
• MGI ID: MGI:5488904
• Synonyms: Arc-CreER, Arc^CreER, ArcCreER, Arc-CreERT2, Arc^CreERT2, ArcCreERT2
• Gene: Arc Location: Chr15:74540932-74544419 bp, - strand Genetic Position: Chr15, 34.25 cM
• Alliance: Arc^{tm1.1(cre/ERT2)Luo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196644
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Arc^{tm1.1(cre/ERT2)Luo} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Arc (MGI:88067)

• Mutation details: A targeting vector was designed to insert a cre/ERT2-SV40 polyA cassette followed by an FRT5-flanked pSV40-NeoR-pA cassette into the the translational start site of the activity regulated cytoskeletal-associated protein gene (Arc). The targeting event displaced the introns and coding regions and replaced the endogenous 3' UTRs (which contribute to mRNA destabilization and to Arc mRNA dendritic trafficking) with an exogenous SV40 polyadenylation signal (to promote high-level expression). All sequences 5' to the translational start site are retained. The resulting chimeric animals were bred to germline-active GFP-FlpO transgenic mice to delete the FRT5-flanked pSV40-NeoR-pA cassette. (J:196644)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Arc (mouse) Summary of all recombinase alleles driven by Arc. Comparative matrix view of recombinase activities Alliance mouse Arc gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Arc Mutation: 28 strains or lines available

References

• Original: J:196644 Guenthner CJ, et al., Permanent genetic access to transiently active neurons via TRAP: targeted recombination in active populations. Neuron. 2013;78(5):773-784
• All: 18 reference(s)