Or8a1^{tm37(Or10j5*,Gnas*)Mom}
Targeted Allele Detail

Summary

• Symbol: Or8a1^{tm37(Or10j5*,Gnas*)Mom}
• Name: olfactory receptor family 8 subfamily A member 1; targeted mutation 37, Peter Mombaerts
• MGI ID: MGI:5489810
• Synonyms: MOR23/M71-RDY-caGs, MOR23(RDY)->M71-caGs-Tomato, Olfr151^{tm37(Olfr16*,-Gnas,-tdTomato)Mom}, Or8a1^{tm37(Olfr16*,Gnas*)Mom}
• Gene: Or8a1 Location: Chr9:37641273-37642357 bp, - strand Genetic Position: Chr9, 20.8 cM
• Alliance: Or8a1^{tm37(Or10j5*,Gnas*)Mom} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233447
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Constitutively active, Inserted expressed sequence)
• Mutations: Insertion, Nucleotide substitutions
• Or8a1^{tm37(Or10j5*,Gnas*)Mom} expresses 2 genes
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Gnas (MGI:95777)	mutated to be constitutively active
  mouse	Or10j5 (MGI:106648)	D121R;R122D mutation

• Mutation details: The endogenous coding sequence was replaced with a mutated Or10j5 sequence, in which the DRY motif was replaced by RDY (D121R;R122D), followed by an internal ribosome entry site and a constitutively active form of Gnas, followed by another internal ribosome entry site and a Tau-tdTomato reporter, in addition to a self-excising neomycin selection cassette (ACNF), thus encoding a tri-cistronic message that has a signaling-deficient OR10J5 olfactory receptor, a constitutively active GNAS to rescue G-protein signaling, and a fluorescent reporter. (J:233447)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Or8a1 Mutation: 69 strains or lines available

References

• Original: J:233447 Movahedi K, et al., Odorant receptors can mediate axonal identity and gene choice via cAMP-independent mechanisms. Open Biol. 2016 Jul;6(7)
• All: 1 reference(s)