T^{tm1(cre/ERT2)Hssk}
Targeted Allele Detail

Summary

• Symbol: T^{tm1(cre/ERT2)Hssk}
• Name: brachyury, T-box transcription factor T; targeted mutation 1, Hiroshi Sasaki
• MGI ID: MGI:5490031
• Synonyms: T^EGFP, T^{nEGFP-CreERT2}
• Gene: T Location: Chr17:8653255-8661328 bp, + strand Genetic Position: Chr17, 4.92 cM
• Alliance: T^{tm1(cre/ERT2)Hssk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194054
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• T^{tm1(cre/ERT2)Hssk} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	T (MGI:98472)

• Mutation details: Recombineering of the BAC clone RP23-99E8 containing the T (brachyury) locus generated a targeting vector designed to replace the stop codon of T and introduce sequences encoding the ERAV 2A peptide, a human histone H2B/EGFP fusion protein (protein is expressed in the cell nucleus), the Thosea asigna virus 2A peptide and cre/ERT2 fusion protein. A self-excising ACN cassette (containing the neo resistance marker) was also included. The vector was electroporated into ES cells for homologous recombination. Chimeras were generated from 3 independent clones (36, 41, and 44) and were bred to C57BL/6J females, with the ACN cassette self-excising during passage through the germline. The line derived from #41 was analyzed further. (J:194054)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	T (mouse) Summary of all recombinase alleles driven by T. Comparative matrix view of recombinase activities Alliance mouse T gene page

Expression

In Mice Carrying this Mutation:

17 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any T Mutation: 59 strains or lines available

References

• Original: J:194054 Imuta Y, et al., Generation of knock-in mice that express nuclear enhanced green fluorescent protein and tamoxifen-inducible Cre recombinase in the notochord from Foxa2 and T loci. Genesis. 2013 Mar;51(3):210-8
• All: 9 reference(s)