Tgfb1^tm1.1Mki
Targeted Allele Detail

Summary

• Symbol: Tgfb1^tm1.1Mki
• Name: transforming growth factor, beta 1; targeted mutation 1.1, Masao Kakoki
• MGI ID: MGI:5490036
• Synonyms: Tgfb1^H, Tgfb1^tm1.1Unc
• Gene: Tgfb1 Location: Chr7:25386427-25404502 bp, + strand Genetic Position: Chr7, 13.98 cM
• Alliance: Tgfb1^tm1.1Mki page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194237
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

Allele Type: Targeted (Inserted expressed sequence) Mutations: Insertion, Intragenic deletion Mutation details: A construct, consisting of a loxP site, the 3' UTR from Fos, two copies of a transcriptional insulator from the chicken beta globin gene, a neo cassette, a second loxP site and the 3' UTR from bovine GH1, replaced the 3' UTR of the gene. Cre mediated recombination removed the 3' UTR from Fos, two copies of a transcriptional insulator from the chicken beta globin gene, and the neo cassette. The mRNA produced by this allele is very stable and results in a higher level of expression compared to wild-type littermates. (J:194237)

Generation of the Tgfb1^tm1Mki and Tgfb1^tm1.1Mki alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tgfb1 Mutation: 35 strains or lines available

References

• Original: J:194237 Kakoki M, et al., Primary aldosteronism and impaired natriuresis in mice underexpressing TGFbeta1. Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5600-5
• All: 2 reference(s)