Spo11^tm2.1Rdco
Targeted Allele Detail

Summary

• Symbol: Spo11^tm2.1Rdco
• Name: SPO11 initiator of meiotic double stranded breaks; targeted mutation 2.1, R Daniel Camerini-Otero
• MGI ID: MGI:5490537
• Synonyms: Spo11^FF, Spo11^YY137,138FF
• Gene: Spo11 Location: Chr2:172819493-172835369 bp, + strand Genetic Position: Chr2, 95.64 cM, cytoband H4
• Alliance: Spo11^tm2.1Rdco page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194067
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 5 was replaced with a modified exon 5 in which nucleotide substitutions result in the amino acid substitutions of phenylalanine for tyrosine at positions 137 and 138 (Y137F, Y138F). Cre-mediated recombination removed the floxed neomycin resistance cassette that was inserted downstream of exon 8. (J:194067)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spo11 Mutation: 21 strains or lines available

References

• Original: J:194067 Boateng KA, et al., Homologous pairing preceding SPO11-mediated double-strand breaks in mice. Dev Cell. 2013 Jan 28;24(2):196-205
• All: 1 reference(s)