Rho^{tm4.1(RHO*/EGFP)Jhw}
Targeted Allele Detail

Summary

• Symbol: Rho^{tm4.1(RHO*/EGFP)Jhw}
• Name: rhodopsin; targeted mutation 4.1, John H Wilson
• MGI ID: MGI:5490865
• Synonyms: P23H, P23H-hRho-GFP
• Gene: Rho Location: Chr6:115908709-115916997 bp, + strand Genetic Position: Chr6, 53.72 cM
• Alliance: Rho^{tm4.1(RHO*/EGFP)Jhw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196863
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Rho^{tm4.1(RHO*/EGFP)Jhw} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  RHO (6010)

  Rho	(MGI:97914)

• Mutation details: The endogenous gene was replaced with an HPRT minigene and a human RHO gene with a P23H mutation and a C terminal fusion to EGFP via homologous recombination. Cre mediated recombination removed the HPRT minigene. Expression levels of the fusion protein are reduced compared to wild-type protein expression and much of what is expressed is degraded. Most of the fusion protein is mislocalized to the inner segments and outer nuclear layer of the retina. (J:196863)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rho Mutation: 51 strains or lines available

References

• Original: J:196863 Price BA, et al., Mislocalization and degradation of human P23H-rhodopsin-GFP in a knockin mouse model of retinitis pigmentosa. Invest Ophthalmol Vis Sci. 2011 Dec;52(13):9728-36
• All: 1 reference(s)