Tg(Rho-Mirc35*)SP1135Kpal
Transgene Detail

Summary

• Symbol: Tg(Rho-Mirc35*)SP1135Kpal
• Name: transgene insertion SP1135, Krzysztof Palczewski
• MGI ID: MGI:5490880
• Synonyms: miR-183/96/182 cluster sponge
• Transgene: Tg(Rho-Mirc35*)SP1135Kpal Location: unknown
• Alliance: Tg(Rho-Mirc35*)SP1135Kpal page

Transgene origin

• Strain of Origin: C57BL/6 x SJL

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The transgene contains a mouse promoter driving photoreceptor cell-specific expression of an EGFP with a 'sponge sequence' inserted into the 3' UTR of EGFP followed by an SV40 polyadenylation sequence. The sponge sequence contains 10 copies of each of sequences complementary to Mir183, Mir96 and Mir182 with mismatches at positions 9 through 12 to enhance stability. Lines SP1231, SP1135 and SP1128 were generated with high, mid and low transgene copy numbers, respectively. Immunohistochemistry does not reliably detect EGFP expression. (J:176614)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:176614 Zhu Q, et al., Sponge transgenic mouse model reveals important roles for the microRNA-183 (miR-183)/96/182 cluster in postmitotic photoreceptors of the retina. J Biol Chem. 2011 Sep 9;286(36):31749-60
• All: 1 reference(s)