Prg4^{tm1(GFP/cre/ERT2)Abl}
Targeted Allele Detail

Summary

• Symbol: Prg4^{tm1(GFP/cre/ERT2)Abl}
• Name: proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein); targeted mutation 1, Andrew Lassar
• MGI ID: MGI:5490969
• Synonyms: Prg4^GFPCreERt2
• Gene: Prg4 Location: Chr1:150325163-150341916 bp, - strand Genetic Position: Chr1, 63.84 cM, cytoband G
• Alliance: Prg4^{tm1(GFP/cre/ERT2)Abl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:197163
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Prg4^{tm1(GFP/cre/ERT2)Abl} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Prg4 (MGI:1891344)

• Mutation details: A targeting vector containing a GFP/cre/ERT2 (green fluorescent protein, cre recombinase and estrogen receptor 1 tamoxifen binding domain fusion gene and an FRT flanked PGK-NEO selection cassette), was inserted into the translation initiation codon of exon 2 of the Prg4, proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein). The resulting chimeric animals were crossed to FLP recombinase expressing mice (ACT-FLPe) mice (on an unknown genetic background) to remove the Frt flanked PGK-neo selection cassette. (J:197163)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Prg4 (mouse) Summary of all recombinase alleles driven by Prg4. Comparative matrix view of recombinase activities Alliance mouse Prg4 gene page

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prg4 Mutation: 71 strains or lines available

Notes

ES cells = unspecified 129/SV

References

• Original: J:197163 Lassar A, Generation of cre knockin allele of Prg4. MGI Direct Data Submission. 2013
• All: 13 reference(s)