Pim1^tm1Mjn
Targeted Allele Detail

Summary

• Symbol: Pim1^tm1Mjn
• Name: proviral integration site 1; targeted mutation 1, Maria Jasin
• MGI ID: MGI:5491066
• Synonyms: Pim1^Drgfp
• Gene: Pim1 Location: Chr17:29709759-29715085 bp, + strand Genetic Position: Chr17, 15.38 cM
• Alliance: Pim1^tm1Mjn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194229
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A hygromycin selection cassette and DR-GFP reporter, were inserted into the locus via homologous recombination. The DR-GFP reporter consists of , consisting of two mutated GFP genes, SceGFP, which is disrupted at an LweI restriction site by the 18-bp recognition site for the I-SceI endonuclease, and iGFP, which is truncated at both the 5' and 3' ends. Following homology directed repair a functional GFP gene expressed under the control of a beta-actin promoter and CMV enhancer is generated. (J:194229)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pim1 Mutation: 16 strains or lines available

References

• Original: J:194229 Kass EM, et al., Double-strand break repair by homologous recombination in primary mouse somatic cells requires BRCA1 but not the ATM kinase. Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5564-9
• All: 5 reference(s)