Gt(ROSA)26Sor^tm18.1Sia
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm18.1Sia
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 18.1, Shinichi Aizawa
• MGI ID: MGI:5491539
• Synonyms: R26-mCherry-hCdt1, Rosa26^{mCherry-hCdt1(30/120)}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm18.1Sia page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196795
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct introduced a floxed STOP cassette followed by an mCherry and the ubiquitylation domains of human CDT1 (amino acids 30 to 120) fusion. Cre-mediated recombination removed the STOP cassette. This fluorescent ubiquitylation-based cell cycle indicator accumulates in the nuclei during G1 phase of the cell cycle. (J:196795)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:196795 Abe T, et al., Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter. Development. 2013 Jan 1;140(1):237-46
• All: 1 reference(s)