Vsig8^{tm1.1(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Vsig8^{tm1.1(KOMP)Mbp}
• Name: V-set and immunoglobulin domain containing 8; targeted mutation 1.1, Mouse Biology Program, UC Davis
• MGI ID: MGI:5493273
• Gene: Vsig8 Location: Chr1:172383505-172391284 bp, + strand Genetic Position: Chr1, 79.95 cM
• Alliance: Vsig8^{tm1.1(KOMP)Mbp} page
• IMPC: Vsig8 gene page

Mutation origin

• Mutant Cell Line: DEPD00552_2_G06
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 2918 starting at position 172387000 and ending at position 172389918 of Chromosome 1 (Genome Build GRCm39). This deletion resulted in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Subsequent cre expression excised the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:157064, J:188991)

Expression

In Mice Carrying this Mutation:	5 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Vsig8 Mutation: 21 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 6 reference(s)