Zfp42^{tm1b(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Zfp42^{tm1b(KOMP)Mbp}
• Name: zinc finger protein 42; targeted mutation 1b, Mouse Biology Program, UC Davis
• MGI ID: MGI:5493322
• Gene: Zfp42 Location: Chr8:43748100-43760017 bp, - strand Genetic Position: Chr8, 23.89 cM
• Alliance: Zfp42^{tm1b(KOMP)Mbp} page
• IMPC: Zfp42 gene page

Mutation origin

• Mutant Cell Line: DEPD00524_3_E01
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 43747816 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 43750063. The critical exon(s) were thus flanked by loxP sites and subsequent cre-mediated recombination excised the critical exon(s) and the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:157064, J:188991)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Zfp42 Mutation: 44 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 6 reference(s)