Mdk^{tm1.1(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Mdk^{tm1.1(KOMP)Mbp}
• Name: midkine; targeted mutation 1.1, Mouse Biology Program, UC Davis
• MGI ID: MGI:5493326
• Gene: Mdk Location: Chr2:91760149-91762348 bp, - strand Genetic Position: Chr2, 50.63 cM
• Alliance: Mdk^{tm1.1(KOMP)Mbp} page
• IMPC: Mdk gene page

Mutation origin

• Mutant Cell Line: DEPD00513_1_C09
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 697 starting at position 91761104 and ending at position 91761801 of Chromosome 2 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Subsequent cre-mediated excision removed the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:157064, J:188991)

Expression

In Mice Carrying this Mutation:	13 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mdk Mutation: 23 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 6 reference(s)