Vamp7^tm1.1Aha
Targeted Allele Detail

Summary

• Symbol: Vamp7^tm1.1Aha
• Name: vesicle-associated membrane protein 7; targeted mutation 1.1, Akihiro Harada
• MGI ID: MGI:5494246
• Gene: Vamp7 Location: unknown Genetic Position: ChrX, Syntenic
• Alliance: Vamp7^tm1.1Aha page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:197602
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Two loxP sites were introduced into introns flanking exons 3 and 4, and an FRT site, a splice acceptor (SA) of Vamp7 exon 5, partial cDNA (exons 5-8) encoding the remainder of the gene, an IRES, a neomyin selection cassette, and a polyA tail followed by the second FRT cassette was inserted between exons 4 and 5. In male mice with the Neo resistance gene in an allele of the Vamp7 gene (Neo/Y), transcription of the Vamp7 gene is expected to be preserved, because the SA traps the Vamp7coding sequence from exons 1 to 4 and Vamp7 cDNA (exons 5-8) follows this to produce an intact transcript (exons 1-8). Recombination of the loxP sites in the germline resulted in the deletion of exons 3 and 4. Western blot analysis indicated that expression of the gene was eliminated in brain, colon, kid- ney, spleen, stomach, small intestine and lung. (J:197602)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
Loading...
hm1	Vamp7^tm1.1Aha/Vamp7^tm1.1Aha	involves: 129S4/SvJae
ot2	Vamp7^tm1.1Aha/Y	involves: 129S4/SvJae

Phenotypes:

Affected Systems	hm1	ot2
show or hide all annotated terms Sex:
cellular	√	√
abnormal axon extension	√	√
nervous system	√	√
abnormal axon extension	√	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vamp7 Mutation: 3 strains or lines available

References

• Original: J:197602 Sato M, et al., The role of VAMP7/TI-VAMP in cell polarity and lysosomal exocytosis in vivo. Traffic. 2011 Oct;12(10):1383-93
• All: 2 reference(s)