Raxtm1.1(rTA,tetO-cre)Lan
Targeted Allele Detail
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Symbol: |
Raxtm1.1(rTA,tetO-cre)Lan |
Name: |
retina and anterior neural fold homeobox; targeted mutation 1.1, Richard A Lang |
MGI ID: |
MGI:5494255 |
Synonyms: |
Rx-tTA:TetOp-Cre |
Gene: |
Rax Location: Chr18:66067710-66072160 bp, - strand Genetic Position: Chr18, 39.03 cM
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Alliance: |
Raxtm1.1(rTA,tetO-cre)Lan page
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Germline Transmission: |
Earliest citation of germline transmission:
J:195568
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Parent Cell Line: |
Other (see notes) (ES Cell)
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Strain of Origin: |
129
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Allele Type: |
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Targeted (Inducible, Recombinase, Transactivator) |
Inducer: |
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doxycycline/tetracycline |
Mutation: |
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Insertion
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Raxtm1.1(rTA,tetO-cre)Lan expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: A cassette encoding an IRES-tTA (tetracycline transactivator) and tetO-cre was inserted into the 3' UTR of the Rax (Rx) gene, 104 bp from the stop codon. A neo resistance marker was included in the cassette. The construct was electroporated into ES cells for homologous recombination. Correctly targeted germline mutants were crossed to flpe-expressing transgenic mice to remove the neo marker. Further breeding to wild-type mice removed the flpe transgene. Removal of doxycycline from the animals' diets permits Rax-dependent cre expression.
(J:195568)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Rax
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Rax Mutation: |
16 strains or lines available
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Original: |
J:195568 Plageman TF Jr, et al., Generation of an Rx-tTA: TetOp-Cre knock-in mouse line for doxycycline regulated Cre activity in the Rx expression domain. PLoS One. 2012;7(11):e50426 |
All: |
1 reference(s) |
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