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Raxtm1.1(rTA,tetO-cre)Lan
Targeted Allele Detail
Summary
Symbol: Raxtm1.1(rTA,tetO-cre)Lan
Name: retina and anterior neural fold homeobox; targeted mutation 1.1, Richard A Lang
MGI ID: MGI:5494255
Synonyms: Rx-tTA:TetOp-Cre
Gene: Rax  Location: Chr18:66067710-66072160 bp, - strand  Genetic Position: Chr18, 39.03 cM
Alliance: Raxtm1.1(rTA,tetO-cre)Lan page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:195568
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  129
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase, Transactivator)
Inducer:    doxycycline/tetracycline
Mutation:    Insertion
 
Raxtm1.1(rTA,tetO-cre)Lan expression driven by 1 gene
 
Mutation detailsA cassette encoding an IRES-tTA (tetracycline transactivator) and tetO-cre was inserted into the 3' UTR of the Rax (Rx) gene, 104 bp from the stop codon. A neo resistance marker was included in the cassette. The construct was electroporated into ES cells for homologous recombination. Correctly targeted germline mutants were crossed to flpe-expressing transgenic mice to remove the neo marker. Further breeding to wild-type mice removed the flpe transgene. Removal of doxycycline from the animals' diets permits Rax-dependent cre expression. (J:195568)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Rax (mouse)
Summary of all recombinase alleles driven by Rax.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rax Mutation:  16 strains or lines available
Notes
ES cells = 129Sv-derived
References
Original:  J:195568 Plageman TF Jr, et al., Generation of an Rx-tTA: TetOp-Cre knock-in mouse line for doxycycline regulated Cre activity in the Rx expression domain. PLoS One. 2012;7(11):e50426
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory