Dbf4^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dbf4^{tm1b(KOMP)Wtsi}
• Name: DBF4 zinc finger; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5494452
• Gene: Dbf4 Location: Chr5:8446973-8472716 bp, - strand Genetic Position: Chr5, 3.43 cM, cytoband A2
• Alliance: Dbf4^{tm1b(KOMP)Wtsi} page
• IMPC: Dbf4 gene page

Mutation origin

• Mutant Cell Line: EPD0396_3_H07
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 8454581 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 8456315. The critical exon(s) were thus flanked by loxP sites, and subsequent Cre-mediated excision removed the critical sequence and neomycin selection cassette resulting in a knockout reporter allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:148605, J:173534, J:188991)

Expression

In Mice Carrying this Mutation:

19 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dbf4 Mutation: 67 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 6 reference(s)