Alb^em12Mvw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Alb^em12Mvw
• Name: albumin; endonuclease-mediated mutation 12, Michael Wiles
• MGI ID: MGI:5494605
• Gene: Alb Location: Chr5:90608756-90624461 bp, + strand Genetic Position: Chr5, 44.7 cM
• Alliance: Alb^em12Mvw page

Mutation origin

• Strain of Origin: B6.Cg-Fcgrt^tm1Dcr Tg(FCGRT)32Dcr/DcrJ

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 4 was targeted by introduction of TALEN mRNA into fertilized B6.Cg-Fcgrt^tm1Dcr Tg(FCGRT)32Dcr/DcrJ oocytes and the resulting founder screened and found to have a 2 base pair deletion beginning at Chromosome 5 base 90,464,113 (GRCm38.p2) which is predicted to result in a frame shift then a premature stop codon 5 amino acids downstream of the encoded frame shift. By ELISA, heterozgyotes have a decrease in serum albumin of approximately 30 percent and homozygotes have no detectable serum albumin. (J:220803)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Alb Mutation: 97 strains or lines available

References

• Original: J:220803 Roopenian DC, et al., Albumin-deficient mouse models for studying metabolism of human albumin and pharmacokinetics of albumin-based drugs. MAbs. 2015;7(2):344-51
• All: 5 reference(s)