Gt(ROSA)26Sor^{tm1(CAG-MAP2K7*/MAPK8,-EGFP)Ftw}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-MAP2K7*/MAPK8,-EGFP)Ftw}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, F Thomas Wunderlich
• MGI ID: MGI:5494696
• Synonyms: JNK^C, ROSA JNK^C
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-MAP2K7*/MAPK8,-EGFP)Ftw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:195844
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-MAP2K7*/MAPK8,-EGFP)Ftw} expresses 2 genes
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MAP2K7 (5609)

  Map2k7

  (MGI:1346871)

  human

  MAPK8 (5599)

  Mapk8

  (MGI:1346861)

• Mutation details: The CAG promoter drives ubiquitous expression of a fusion of human MAP2K7 containing the constitutively activating mutations aspartic acid for serine and threonine at positions 271 and 275, respectively (S271D and T275D) and MAPK8 followed by an FRT-flanked IRES sequence and EGFP gene. A floxed neomycin resistance and STOP cassette prevents expression of the fusion product until cre-mediated removal. (J:195844)

Generation of the Gt(ROSA)26Sor^{tm1(CAG-MAP2K7*/MAPK8,-EGFP)Ftw} allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:195844 Pal M, et al., Alteration of JNK-1 signaling in skeletal muscle fails to affect glucose homeostasis and obesity-associated insulin resistance in mice. PLoS One. 2013;8(1):e54247
• All: 3 reference(s)