Gt(ROSA)26Sor^{tm4(EWSR1/ATF1)Mrc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(EWSR1/ATF1)Mrc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Mario R Capecchi
• MGI ID: MGI:5495288
• Synonyms: Gt(ROSA)26Sor^{tm2(EWSR1/ATF1)Mrc}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(EWSR1/ATF1)Mrc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:194308
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm4(EWSR1/ATF1)Mrc} expresses 2 genes
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ATF1 (466)

  Atf1	(MGI:1298366)
  Atf1-ps	(MGI:3037720)

  human

  EWSR1 (2130)

  Ewsr1

  (MGI:99960)

• Mutation details: A cDNA clone was produced using RNA purified from a confirmed "clear cell sarcoma" tumor. The cDNA contained the EWSR1/ATF1 fusion product of the t(12;22) chromosomal translocation typical of these tumors. This cDNA was inserted downstream of a floxed pgk-neomycin cassette and upstream of an IRES-EGFP. Stop codons in the neomycin cassette prevent EWSR1/ATF1 and EGFP expression. (J:194308)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:194308 Straessler KM, et al., Modeling clear cell sarcomagenesis in the mouse: cell of origin differentiation state impacts tumor characteristics. Cancer Cell. 2013 Feb 11;23(2):215-27
• All: 3 reference(s)