Dlg4^tm1.1Tdoi
Targeted Allele Detail

Summary

• Symbol: Dlg4^tm1.1Tdoi
• Name: discs large MAGUK scaffold protein 4; targeted mutation 1.1, Tomoko Doi
• MGI ID: MGI:5495768
• Synonyms: 1d2d-PSD-95-EGFP
• Gene: Dlg4 Location: Chr11:69908029-69938107 bp, + strand Genetic Position: Chr11, 42.96 cM, cytoband B4
• Alliance: Dlg4^tm1.1Tdoi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:197875
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exons 5 through 8 were replaced with a modified full-length cDNA and EGFP fusion followed by a floxed neomycin resistance cassette. The modified cDNA contains the mutations S78N (AGC to AAC), A80V (GCA to GTA), S183N (AGC to AAC), and A185V (GCA to GTA), and the deletions T83 to I88 and V178 to I183 that mutate PDZ1/2 to resemble PDZ3. Cre-mediated recombination removed the neomycin resistance cassette. (J:197875)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dlg4 Mutation: 44 strains or lines available

References

• Original: J:197875 Nagura H, et al., Impaired synaptic clustering of postsynaptic density proteins and altered signal transmission in hippocampal neurons, and disrupted learning behavior in PDZ1 and PDZ2 ligand binding-deficient PSD-95 knockin mice. Mol Brain. 2012;5:43
• All: 1 reference(s)