Phf2^tm1.1Yima
Targeted Allele Detail

Summary

• Symbol: Phf2^tm1.1Yima
• Name: PHD finger protein 2; targeted mutation 1.1, Yuuki Imai
• MGI ID: MGI:5496656
• Synonyms: Phf2^Z
• Gene: Phf2 Location: Chr13:48955226-49024361 bp, - strand Genetic Position: Chr13, 24.96 cM, cytoband A3-A4
• Alliance: Phf2^tm1.1Yima page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198329
• Parent Cell Line: M1/M-1 (ES Cell)
• Strain of Origin: (129 x C57BL/6)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The construct targeted upstream of exon 6 contains (5' to 3') a loxP site, lacZ gene, FRT site, neomycin resistance cassette, loxP site and FRT site. An additional FRT site was inserted downstream of exon 6. Flp-mediated recombination removed the neomycin resistance cassette and exon 6 but left the lacZ gene intact. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts. (J:198329)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Phf2 Mutation: 46 strains or lines available

References

• Original: J:198329 Okuno Y, et al., Epigenetic regulation of adipogenesis by PHF2 histone demethylase. Diabetes. 2013 May;62(5):1426-34
• All: 1 reference(s)